Notice was served by the owners on Wednesday, August 17 requiring all bus service operations to cease at the site by Friday, September 16.
The council has been engaging with the owners, the Generator Group, to identify and assess alternative locations for the provision of a bus station as part of their planning application to the South Downs National Park Authority to develop the current site.
Cllr Claire Dowling, lead member for transport and the environment, said: “We are extremely disappointed at the owner’s decision to serve the council notice.
“We remain committed to working with them to deliver an alternative location for a town centre bus station, as they are required to do to satisfy the relevant policy within the South Downs National Park Local Plan.
“As a potential site has been identified we hope they will proceed with funding its development as a matter of urgency to limit the disruption to bus passengers, businesses, and residents in Lewes.”
Alternative arrangements for temporary bus stops around the town centre will be put in place to ensure bus operators can continue to serve passengers.
The temporary bus stops will be located at Friars Walk and on School Hill and Eastgate Street, with layover facilities at Little East Street.
Further information on the temporary bus stop locations is available at https://www.eastsussex.gov.uk/roads-transport/public/buses/lewes-bus-station-where-to-catch-your-bus